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CyTOF 2 Maintenance

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CyTOF 2 Maintenance1 hour

Removal, Cleaning and Reinstallation of the Cones

  • Step 1

    Remove the Torch Assembly prior to beginning this procedure.

    Open the instrument front access door.

  • Step 2

    The cone removal tool has two ends. One end is used to remove the Sampler Cone, and the other to remove the Skimmer-Reducer.

  • Step 3

    The Sampler Cone has four holes on the face. Insert the cone removal tool into the two smooth (non-threaded) holes.

  • Step 4

    Rotate the cone removal tool and pull towards you to release the Sampler Cone from the vacuum interface. Remove the Sampler Cone from the tool and set aside.

    Note: You may need to use isopropanol to lubricate.

  • Step 5

    Insert the other end of the cone removal tool into the two holes on the Skimmer-Reducer Assembly. Turn the tool counterclockwise to release the cones from the vacuum interface.

    Remove the Skimmer Reducer from the tool.

  • Step 6

    Stack the cones as follows: First stack the bottom adapter, then the Sampler Cone, followed by the top adapter. The Skimmer-Reducer Assembly rests on top with the screws facing up.

  • Step 7

    Fill the container with 10% Citranox® and sonicate for 15 minutes. Place the stack in the cone cleaning container one part at a time. Sonicate for 15 minutes.

    Pour off the Citranox and fill with DIW. Replace the stack. Sonicate for an additional 3-4 minutes.

    Repeat this two times. Rinse with isopropanol. Dry with a blow dryer.

  • Step 8

    Place the Skimmer‐Reducer assembly on the side of the cone removal tool with two magnets.

  • Step 9

    Coat the threads of the Skimmer-Reducer Assembly with graphite using a No. 2 pencil.
  • Step 10

    Place the cone removal tool flush into the interface and begin to turn clockwise. Continue to turn clockwise until the Skimmer-Reducer Assembly is firmly seated in the interface. Detach the cone removal tool.

  • Step 11

    Place the other side of the cone removal tool in the smooth (non-threaded) holes of the Sampler Cone. Place the cone flush into the interface and begin to turn clockwise. You may need to lubricate the O-ring with a drop of isopropanol. Turn the cone clockwise while applying gentle pressure.

    Remove the cone removal tool. Press the cone to make sure it is firmly seated.

    Follow this procedure with the reassembly of the torch.