1550 results found for "data analysis"
Singular Analysis Toolset
An innovative, open-source solution for analyzing and visualizing data, the Singular™ Analysis Toolset offers powerful methods for identifying gene expression and mutation patterns at the single-cell level. Designed for use with Biomark and C1 systems.
Download Software: Singular Analysis Toolset Software
User Guide: Singular Analysis Toolset User Guide
Release Notes: Singular Analysis Toolset Release Notes
Instructions: Singular Analysis Toolset Practice Sets Instructions
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faq
What types of data can be analyzed for gene expression by the Singular Analysis Toolset?
The Singular Analysis Toolset can analyze data from gene expression experiments based on either mRNA sequencing or qPCR. …
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faq
I detected thousands of genes in my RNA sequencing data. Why does the Singular Analysis Toolset display only a limited number of them?
By default, the Singular Analysis Toolset will display a limited number of genes, ranked by their principal component analysis score. …
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faq
What data analysis and bioinformatic pipeline tools are available?
If you already have a pipeline, information regarding the Advanta™ CFTR NGS Library Prep Assay design can be provided to support pipeline configuration. If you need a pipeline, Fluidigm is vetting commercially available analysis software from various third parties. Contact your Fluidigm Account Executive to discuss requirements. …
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How does the Biomark Data Analysis software calculate ddCt?
The relative expression of the target gene (delta delta Ct, or ddCt) requires the assignment of one or more housekeeping genes, which are assumed to be uniformly and constantly expressed in all samples, as well as one or more reference samples. …
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How does the Fluidigm SNP Genotyping Analysis software determine the genotype call for a sample?
Allele discrimination is primarily based on ratios from the two normalized probe measurements. …
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What analysis approaches have been applied to (published) mass cytometry data?
High-dimensional mass cytometry data can be analyzed using many different methods. …
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What is the difference between NO CALL and an Invalid call in the Fluidigm Genotyping Analysis software?
A NO CALL is assigned by the genotype-calling algorithm when, based on the confidence threshold set by the user, it cannot associate a given datapoint with a cluster. …
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faq
What is principal component analysis (PCA)?
PCA is a statistical method to reduce data containing a large number of variables into as few uncorrelated variables, or principal components, as possible. …
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When should I use the All SNP Scatter Plot View option in the SNP Genotyping Analysis software?
The All SNP Scatter Plot View option displays all SNP data from the IFC in a single scatter plot. …
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faq
What analysis tools are available to help explore and understand CyTOF® 2 mass cytometry data?
Mass cytometry data can be conveniently analyzed using FCS 3.0-compatible software. …
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faq
Can I combine multiple IFC runs in the Real-Time PCR Analysis software?
IFC runs with different assay sets can be combined as long as they have the same sample names. …
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faq
How many biological replicates (cells) should be used for single-cell analysis on the C1 system?
The ability to identify significant differences in a single-cell experiment depends not only on the number of cells analyzed but also on other factors, including the degree of variation within the population analyzed, the number of genes assayed, and the ability of those genes to differentiate population variation. …
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What is the difference between SNP Type Normalization and no template control (NTC) normalization?
SNP Type Normalization is the default normalization method for Fluidigm SNP Type assays. …
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faq
What is the basic method for generating hierarchical clusters?
Hierarchical clustering groups data in the form of a heat map that successively merges similar groups or data points. …
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faq
Do you provide any software for single-cell gene expression analysis?
The Singular Analysis Toolset from Fluidigm provides a set of software functions for higher-level expression analysis using R, but it is not appropriate for bulk gene expression analysis. …
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faq
What can I do to help improve call rates?
Genotype call quality can vary when the amount or the quality of the input DNA is not ideal, potentially leading to either an incorrect genotype call or a NO CALL for a particular SNP. …
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faq
Can I run Fluidigm data analysis software on a Mac?
Fluidigm Genotyping and Gene Expression data analysis software can be run on a Mac only if a Windows emulation program is used. …
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article
Unlocking Topographic Biomarkers
The interactive histoCAT toolbox unifies the cytometry and imaging communities …
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faq
Where can I view the data that has been excluded from variant analysis?
In the mult_allele_data file, you can see the data that has been excluded from variant analysis. …
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faq
How should I perform data analysis for the Advanta IO Gene Expression Assay?
Use the Real Time PCR Analysis software (version 4.5.1 or higher) to conduct primary data analysis. …