Histone H3, the most widely modified of all 4 nucleosomal histones, is phosphorylated on threonine 3, serine 10, serine 28 and threonine 32 when cells enter mitosis. Phosphorylation at serine 10 or 28 occurs in association with the induction of immediate-early (IE) genes and is part of the nucleosomal response downstream of the activation of the ERK1/2 or p38 MAPK pathways. As a downstream target of MAPK signaling pathways, H3 phosphorylation is a response to a vast array of extracellular stimuli including growth factors, stressors such as UV light, alcohol and neurotransmitters. During mitosis, Aurora B and PP1 phosphorylate and dephosphorylate H3 serine 10 and serine 28, respectively.
Anti-pHistone H3 [Ser28] (HTA28)-176Yb—25 µg
Target:pHistone H3 [Ser28]