The Maxpar Direct Immune Profiling Assay is designed and optimized for deep immune profiling of human peripheral whole blood and PBMC.
The 30 antibodies in the kit are provided in a dry single-tube format that includes Cell-ID Intercalator-103Rh for identification of live/dead cells. The assay enables comprehensive identification and characterization of 37 immune cell populations, including all major T cell subsets (CD4+ and CD8+ naive, central memory, effector memory, and terminal effector), CD4+ regulatory T cells, CD4– mucosal-associated invariant T cells (MAIT) / natural killer T (NKT) cells, B cell subsets (naive and memory, plasmablasts), natural killer (early and late) cells, T helper (Th) cell phenotypic subsets (Th1-like, Th2-like, and Th17-like), gamma delta (γδ) T cells, monocytes (classical, transitional, and nonclassical), dendritic cell subsets (plasmacytoid and myeloid), granulocytes, basophils, eosinophils, and neutrophils. The assay kit includes all Fluidigm reagents needed for cell staining. Additional reagents needed to run a mass cytometry experiment on the Helios™ system can be purchased separately from Fluidigm or third-party suppliers.
- Cell-ID™ Intercalator-Ir—125 μM, 25 μL
- Maxpar® Dry Antibody Panel*—25 tests
- Maxpar Cell Staining Buffer—500 mL
- Maxpar Fix and Perm Buffer—25 mL
- Maxpar PBS—100 mL
- Maxpar Cell Acquisition Solution—200 mL†
* Antibodies in pre-mixed dry cocktail with Cell-ID Intercalator-103Rh.
† This solution is for use in sample acquisition only.
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