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Maxpar® Direct™ Immune Profiling Assay™

The Maxpar Direct Immune Profiling Assay is designed and optimized for deep immune profiling of human peripheral whole blood and PBMC.

The 30 antibodies in the kit are provided in a dry single-tube format that includes Cell-ID Intercalator-103Rh for identification of live/dead cells. The assay enables comprehensive identification and characterization of 37 immune cell populations, including all major T cell subsets (CD4+ and CD8+ naive, central memory, effector memory, and terminal effector), CD4+ regulatory T cells, CD4– mucosal-associated invariant T cells (MAIT) / natural killer T (NKT) cells, B cell subsets (naive and memory, plasmablasts), natural killer (early and late) cells, T helper (Th) cell phenotypic subsets (Th1-like, Th2-like, and Th17-like), gamma delta (γδ) T cells, monocytes (classical, transitional, and nonclassical), dendritic cell subsets (plasmacytoid and myeloid), granulocytes, basophils, eosinophils, and neutrophils. The assay kit includes all Fluidigm reagents needed for cell staining. Additional reagents needed to run a mass cytometry experiment on the Helios™ system can be purchased separately from Fluidigm or third-party suppliers.

Kit Contents

  • Cell-ID™ Intercalator-Ir—125 μM, 25 μL
  • Maxpar® Dry Antibody Panel*—25 tests
  • Maxpar Cell Staining Buffer—500 mL
  • Maxpar Fix and Perm Buffer—25 mL
  • Maxpar PBS—100 mL
  • Maxpar Cell Acquisition Solution—200 mL

* Antibodies in pre-mixed dry cocktail with Cell-ID Intercalator-103Rh.
This solution is for use in sample acquisition only.

Antibody (clone)

Mass

CD45 (HI30) 89Y
CD196 /CCR6 (G034E3) 141Pr
CD123 (6H6) 143Nd
CD19 (HIB19) 144Nd
CD4 (RPA-T4) 145Nd
CD8a (RPA-T8) 146Nd
CD11c (Bu15) 147Sm
CD16 (3G8) 148Nd
CD45RO (UCHL1) 149Sm
CD45RA (HI100) 150Nd
CD161 (HP-3G10) 151Eu
CD194/CCR4 (L291H4) 152Sm
CD25 (BC96) 153Eu
CD27 (O323) 154Sm
CD57 (HCD57) 155Gd
CD183/CXCR3 (G025H7) 156Gd
CD185/CXCR5 (J252D4) 158Gd
CD28 (CD28.2) 160Gd
CD38 (HB-7) 161Dy
CD56/NCAM (NCAM16.2) 163Dy
TCRγδ (B1) 164Dy
CD294 (BM16) 166Er
CD197/CCR7 (G043H7) 167Er
CD14 (63D3) 168Er
CD3 (UCHT1) 170Er
CD20 (2H7) 171Yb
CD66b (G10F5) 172Yb
HLA-DR (LN3) 173Yb
IgD (IA6-2) 174Yb
CD127 (A019D5) 176Yb
Cell-ID Intercalator-103Rh 103Rh
  • Species:
    Human

 

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