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FAQs

Do I need a special kit or IFC to run TCR sequencing on the C1?

No. The single-cell T cell receptor (TCR) sequencing application described on Script Hub™ uses data produced by the C1 Single-Cell mRNA Seq Protocol (PN 100‑7168), which provides whole transcriptome amplification with full-length mRNA transcripts. The protocol uses the existing C1 mRNA Seq IFC and reagent kits, and the resulting data is analyzed using the TraCeR analysis tool. Recommended sequencing depth is 1 million reads per T cell.