How should I dilute preamplification products after amplification?
To help protect against degradation during storage, preamplification products should be diluted in a DNase/RNase-free, low-EDTA TE buffer (10 mM Tris, 0.1 mM EDTA; pH 8.0) after preamplification. Dilution in PCR Certified Water is acceptable if preamplification products do not require long-term storage. A 1:5 dilution is recommended for TaqMan genotyping, and a 1:100 dilution for SNP Type Assay genotyping. However, this dilution factor may require optimization for best results.