Can I combine multiple IFC runs in the Real-Time PCR Analysis software?
IFC runs with different assay sets can be combined as long as they have the same sample names. For more information see the documents Biomark HD Data Collection User Guide (PN 100-2451) and Biomark/EP1 Data Collection User Guide (PN 68000127).
To analyze multiple IFC runs together, or anytime data between IFCs is being compared, we recommend calculating ddCt. This value should be calculated from a standard sample that is included on every IFC in the dataset.