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FAQs

How does the Biomark Data Analysis software calculate ddCt?

The relative expression of the target gene (delta delta Ct, or ddCt) requires the assignment of one or more housekeeping genes, which are assumed to be uniformly and constantly expressed in all samples, as well as one or more reference samples. The software calculates dCt within each reference and sample by normalizing the Ct of each gene to the Ct of the chosen housekeeping gene. ddCt is then calculated by subtracting the dCt of the reference from the dCt of the sample.