What is the difference between EvaGreen and TaqMan?
TaqMan® assays (Thermo Fisher Scientific) are primer and probe sets that are designed to individual genes and provide very specific detection of gene expression.
EvaGreen® is a double-stranded DNA binding dye that is less inhibitory to PCR and less toxic than SYBR™ Green. DNA binding dyes will detect any double-stranded product including the gene of interest and any off-target amplicons or primer dimers. Gene expression using double-stranded binding dyes is typically much less expensive and more flexible than TaqMan chemistry.