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FAQs

What is provided with my order of Delta Gene assays?

Mixed forward and reverse primers in 96-well plates. Forward and reverse primers are mixed in equal molar ratios and normalized to 100 μM in 100 μL, where each primer pair is provided in nuclease-free water in a final volume of 100 μL.

Customer Informatics Packet:

  • The Design File, which is a Microsoft® Excel® file that includes assay name, Fluidigm Assay ID, primer sequences, representative RefSeq, gene symbol, gene aliases, gene full name and gene ontology: function, process and component
  • A plate layout file containing positions of the assays in 96-well plate format
  • A PDF assay report that details the assay names, NCBI Reference Sequence ID (RefSeq), image of primer binding positions, amplicon length and gene ontology information
  • (For wet-lab tested assays only) An assay performance report including mean dCt slope for each assay