What factors does D3 assay design consider when designing primers for Access Array?
Access Array Target-Specific Primers are designed with proprietary primer design software. This software provides optimal target-specific primer pairs that avoid nonspecific amplification. We require customers to review their primer designs before placing an order. Primer design considerations by Fluidigm Bioinformatics include but are not limited to:
- Primer and amplicon specificity
- Avoiding placing primers over SNPs where they could impact specificity
- Avoiding placing primers on highly structured GC-rich regions or repeats
- Primer melting temperatures within an order being typically ±2 °C where possible