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Multiplex Quantitative Measurement of mRNAs from Fixed Tissue Microarray Sections

Armani, M., Tangrea, M., Yang, B. et al.

The development of prognostic and diagnostic biomarkers, such as those from gene expression studies, requires independent validation in clinical specimens. Immunohistochemistry on tissue microarrays (TMAs) of formalin-fixed paraffin-embedded (FFPE) tissue is often used to increase statistical power, and it is used more often than in situ hybridization (ISH), which can be technically limiting. Here we introduce a method for performing quantitative gene expression analysis across a TMA using an adaptation of 2D-RT-qPCR, a recently developed technology for measuring transcript levels in a histological section while maintaining two-dimensional positional information of the tissue sample. As a demonstration of utility, a TMA with tumor and normal human prostate samples was used to validate expression profiles from previous array-based gene discovery studies of prostate cancer. The results show that 2D-RT-qPCR expands the utility of TMAs to include sensitive and accurate gene expression measurements.

Citation

Armani, M., Tangrea, M., Yang, B. et al. "Multiplex Quantitative Measurement of mRNAs from Fixed Tissue Microarray Sections" Applied Immunohistochemistry and Molecular Morphology (2015): 323-30