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Microfluidic single-cell whole-transcriptome sequencing

Streets, A.M., Zhang, X., Cao, C. et al.

RNA sequencing of single cells enables measurement of biological variation in heterogeneous cellular populations and dissection of transcriptome complexity that is masked in ensemble measurements of gene expression. The low quantity of RNA in a single cell, however, hinders efficient and consistent reverse transcription and amplification of cDNA, limiting accuracy and obscuring biological variation with high technical noise. We developed a microfluidic approach to prepare cDNA from single cells for high-throughput transcriptome sequencing. The microfluidic platform facilitates single-cell manipulation, minimizes contamination, and furthermore, provides improved detection sensitivity and measurement precision, which is necessary for differentiating biological variability from technical noise.

Citation

Streets, A.M., Zhang, X., Cao, C. et al. "Microfluidic single-cell whole-transcriptome sequencing" Proceedings of the National Academy of Sciences of the United States of America (2014): 7,048–53