Microfluidic device reads up to four consecutive base pairs in DNA sequencing-by-synthesis
Kartalov, E.P., Quake, S.R.
We have developed the first fully integrated microfluidic system for DNA sequencing-by-synthesis. Using this chip and fluorescence detection, we have reliably sequenced up to 4 consecutive bps. The described sequencer can be integrated with other microfluidic components on the same chip to produce true lab-on-a-chip technology. The surface chemistry that was designed to anchor the DNA to elastomeric microchannels is useful in a broad range of studies and applications.
Kartalov, E.P., Quake, S.R. "Microfluidic device reads up to four consecutive base pairs in DNA sequencing-by-synthesis" Nucleic Acids Research (2004): 2,873–9