Mass cytometric analysis of HIV entry, replication, and remodeling in tissue CD4+ T cells
Cavrois, M., Banerjee, T., Mukherjee, G. et al.
To characterize susceptibility to HIV infection, we phenotyped infected tonsillar T cells by single-cell mass cytometry and created comprehensive maps to identify which subsets of CD4+ T cells support HIV fusion and productive infection. By comparing HIV-fused and HIV-infected cells through dimensionality reduction, clustering, and statistical approaches to account for viral perturbations, we identified a subset of memory CD4+ T cells that support HIV entry but not viral gene expression. These cells express high levels of CD127, the IL-7 receptor, and are believed to be long-lived lymphocytes. In HIV-infected patients, CD127-expressing cells preferentially localize to extrafollicular lymphoid regions with limited viral replication. Thus, CyTOF-based phenotyping combined with analytical approaches to distinguish between selective infection and receptor modulation by viruses can be used as a discovery tool.
Cavrois, M., Banerjee, T., Mukherjee, G. et al. "Mass cytometric analysis of HIV entry, replication, and remodeling in tissue CD4+ T cells" Cell Reports (2017): 984–98