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Lymphoid priming in human bone marrow begins prior to CD10 expression with upregulation of L-selectin

Kohn, L.A., Hao, Q.L., Sasidharan, R., Parekh, C., Ge, S., Zhu, Y., Mikkola, H.K.A., Crooks, G.M.

The expression of CD10 has long been used to define human lymphoid commitment. We report a unique lymphoid-primed population in human bone marrow that was generated from hematopoietic stem cells (HSCs) before the onset of CD10 expression and B cell commitment. This subset was identified by high expression of the homing molecule L-selectin (CD62L). CD10CD62Lhi progenitors possessed full lymphoid and monocytic potential, but lacked erythroid potential. Gene expression profiling placed the CD10CD62Lhi population at an intermediate stage of differentiation between HSCs and lineage-negative (Lin) CD34+CD10+ progenitors. L-selectin was expressed on immature thymocytes and its ligands were expressed at the cortico-medullary junction, suggesting a possible role in thymic homing. These studies identify the earliest stage of lymphoid priming in human bone marrow.

Citation

Kohn, L.A., Hao, Q.L., Sasidharan, R., Parekh, C., Ge, S., Zhu, Y., Mikkola, H.K.A., Crooks, G.M. "Lymphoid priming in human bone marrow begins prior to CD10 expression with upregulation of L-selectin" Nature Immunology (2012): 963–71