Intergrative Gene Regulatory Network Analysis Reveals Light-Induced Regional Gene Expression Phase Shift Program
Zhu, H., Vadigepalli, R., Rafferty, R., Gonye, G.E., Weaver, D.R., Schwaber, J.S.
Intergrative Gene Regulatory Network analysis Reveals Light-Induced Regional Gene Expression Phase Shift Programs in the Mouse Suprachiasmatic Nucleus
We use the multigenic pattern of gene expression across suprachiasmatic nuclei (SCN) regions and time to understand the dynamics within the SCN in response to a circadian phase-resetting light pulse. Global gene expression studies of the SCN indicate that circadian functions like phase resetting are complex multigenic processes. While the molecular dynamics of phase resetting are not well understood, it is clear they involve a “functional gene expression program”, e.g., the coordinated behavior of functionally related genes in space and time. In the present study we selected a set of 89 of these functionally related genes in order to further understand this multigenic program. By use of high-throughput qPCR we studied 52 small samples taken by anatomically precise laser capture from within the core and shell SCN regions, and taken at time points with and without phase resetting light exposure. The results show striking regional differences in light response to be present in the mouse SCN. By using network-based analyses, we are able to establish a highly specific multigenic correlation between genes expressed in response to light at night and genes normally activated during the day. The light pulse triggers a complex and highly coordinated network of gene regulation. The largest differences marking neuroanatomical location are in transmitter receptors, and the largest time-dependent differences occur in clock-related genes. Nighttime phase resetting appears to recruit transcriptional regulatory processes normally active in the day. This program, or mechanism, causes the pattern of core region gene expression to transiently shift to become more like that of the shell region.
Zhu, H., Vadigepalli, R., Rafferty, R., Gonye, G.E., Weaver, D.R., Schwaber, J.S. "Intergrative Gene Regulatory Network Analysis Reveals Light-Induced Regional Gene Expression Phase Shift Program" PLoS One (2012): e37833