High-throughput spatial mapping of single-cell RNA-seq data to tissue of origin
Achim, K., Pettit, J.B., Saraiva, L.R. et al.
Understanding cell type identity in a multicellular organism requires the integration of gene expression profiles from individual cells with their spatial location in a particular tissue. Current technologies allow whole-transcriptome sequencing of spatially identified cells but lack the throughput needed to characterize complex tissues. Here we present a high-throughput method to identify the spatial origin of cells assayed by single-cell RNA-sequencing within a tissue of interest. Our approach is based on comparing complete, specificity-weighted mRNA profiles of a cell with positional gene expression profiles derived from a gene expression atlas. We show that this method allocates cells to precise locations in the brain of the marine annelid Platynereis dumerilii with a success rate of 81%. Our method is applicable to any system that has a reference gene expression database of sufficiently high resolution.
Achim, K., Pettit, J.B., Saraiva, L.R. et al. "High-throughput spatial mapping of single-cell RNA-seq data to tissue of origin" Nature Biotechnology (2015): 503–9