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Quality RNA Means Better Cancer Research

Improve profiling of precious samples using the Advanta FFPE RNA Extraction Kit

Feature

Advanta IO Gene Expression Assay

Tumor gene expression profiling in cancer research is an effective tool for measuring immune response during cancer progression and treatment studies. As emerging therapies reveal new biomarkers and expand the need for sample testing, the costs and labor required to complete this important work also increase.


Cancer researchers now have a reliable, sensitive and cost-effective tool for identifying gene expression signatures from immune and cancer cells: The Advanta™ IO Gene Expression Assay is designed for use with the Biomark™ HD system. For optimized gene detection, pair it with the Advanta™ FFPE RNA Extraction Kit.


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Improving the process

Processing FFPE samples can be challenging due to limited sample quantities or fragmented RNA transcripts caused by nucleic acid degradation as a result of tissue fixation and storage methods. In addition, most extraction kits rely on column purification to remove RNA and DNA, causing significant nucleic acid loss during the isolation process. To address this issue, the Advanta FFPE RNA Extraction Kit uses a column-free approach to effectively recover quality RNA from FFPE samples.

Download our product flyer to learn more about using Advanta FFPE RNA Extraction Kit to accelerate your investigative research.

The Advanta FFPE RNA Extraction Kit stands apart from other commercial kits by providing:

  • A single-tube extraction purification process
  • Enhanced safety with xylene-free paraffin removal
  • More precious samples are qualified for downstream analysis
  • Improved detection during gene expression analysis

“Sirona Dx specializes in expression profiling of challenging FFPE samples and we welcomed the opportunity to partner with Fluidigm to develop an improved RNA extraction methodology. To date we have processed several hundred samples with the Advanta FFPE RNA Extraction Kit, and our pharma clients have been astonished by the quality of RNA extracted and delighted at our ability to interrogate more of their precious FFPE samples.” —Nasry Yassa, CEO, Sirona Dx

Sirona Dx case study

Clinical research services provider Sirona Dx, Inc., based in Vancouver, Washington, performed gene expression analysis of FFPE samples extracted using the Advanta FFPE RNA Extraction Kit and a leading column-based commercial kit. After extraction, the group reverse-transcribed and preamplified the RNA and analyzed the cDNA on Biomark HD using the Advanta IO Gene Expression Assay panel containing 170 unique biomarkers for profiling tumor immunobiology and identification. Gene expression analysis data demonstrated more target gene detection in samples extracted using the Advanta FFPE RNA Extraction Kit. These samples typically showed lower cycle threshold values than those extracted using the alternative kit.

“Sirona Dx specializes in expression profiling of challenging FFPE samples and we welcomed the opportunity to partner with Fluidigm to develop an improved RNA extraction methodology,” said Sirona Dx CEO Nasry Yassa. “To date we have processed several hundred samples with the Advanta FFPE RNA Extraction Kit, and our pharma clients have been astonished by the quality of RNA extracted and delighted at our ability to interrogate more of their precious FFPE samples­.”

Contact us to learn more about the Advanta FFPE RNA Extraction Kit.

For Research Use Only. Not for use in diagnostic procedures.